Advantages of 'Serial Dilutions'. This section is not a recipe for your experiment. It explains some principles for designing dilutions that give optimal results. Oct 8, 2013 - Fortunately, through precise serial dilution of a sample, it is possible to get down to a number that is much easier to work with.
A serial dilution is the stepwise of a in. Usually the at each step is constant, resulting in a of the in a fashion.
A ten-fold serial dilution could be 1, 0.1 M, 0.01 M, 0.001 M. Serial dilutions are used to accurately create highly diluted solutions as well as solutions for resulting in with a. A tenfold dilution for each step is called a logarithmic dilution or log-dilution, a 3.16-fold (10 0.5-fold) dilution is called a half-logarithmic dilution or half-log dilution, and a 1.78-fold (10 0.25-fold) dilution is called a quarter-logarithmic dilution or quarter-log dilution. Serial dilutions are widely used in experimental sciences, including,,,.
Contents • • • • • In biology and medicine [ ] In and, besides the more conventional uses described above, serial dilution may also be used to reduce the concentration of microscopic organisms or cells in a sample. As, for instance, the number and size of that grow on an plate in a given time is concentration-dependent, and since many other diagnostic techniques involve physically counting the number of micro-organisms or cells on specials printed with grids (for comparing concentrations of two organisms or cell types in the sample) or wells of a given volume (for absolute concentrations), dilution can be useful for getting more manageable results.
Serial dilution is also a cheaper and simpler method for preparing than. In homeopathy [ ]. Experiments in Microbiology, Plant Pathology and Biotechnology. New Age Publishers, 2005, p. • Booth, C.; et al. 
Methods in microbiology 35. Academic Press. • Weissmann, Gerald (2006). The FASEB Journal. 20 (11): 1755–1758.. Retrieved 2008-02-01. • Ernst, Edzard (November 2005).
'Is homeopathy a clinically valuable approach?' Trends in Pharmacological Sciences. 26 (11): 547–548.. Bishop, Edward P. Fody, Larry E. Clinical Chemistry: Principles, Procedures, Correlations.
Lippincott Williams & Wilkins, 2004, p. 24. External links [ ] •, Bates College.
Dilution is the act of mixing a chemical with other substance, usually distilled water to make it lighter in composition. It is usually done if the chemical concentration is too high than the desired composition. Thus, serial dilution simply means a series of repeated dilution performed on the same chemical basically to change its concentration.
After performing the dilution, we need to know how much difference are the diluted chemical and the initial, undiluted ones. One way is by obtaining a factor called the Dilution Factor (DF). The factor can be obtained by dividing the actual volume of the initial chemical used to the final volume of the chemical after water is added. As example, if 1.0 ml of 3M HCl is added with 9 ml of distilled water, the dilution factor becomes 1/10. In serial dilution, the entire dilution factor will be multiple together to be the actual dilution factor. There are several benefits of performing serial dilution.
Serial dilution comes in handy when the solution is too concentrated to be used in experiments or ingredients preparation. This is to ensure that the exact concentration can be obtained for the experiment to become success.
Other than that, the diluted solution from this serial dilution can be used to count the concentration of the actual solution. By knowing the dilution factor of certain solution, the calculations of the concentration become easier and systematic.
This method is applicable in several fields, not only in chemistry. Serial dilution is a simple yet efficient technique to determine the number of cells or organisms in a concentrated sample. First, take a portion of the sample and does serial dilution on it.
Repeat the steps until the cells can be observed under the microscope when the diluted sample was observed. Then, count the dilution factor and times it with the actual volume of the sample. Other than that, medicine administration require suitable dose for each patient with variable needs. This is where serial dilution is useful. References 1. Colin Ratledge, Bjorn Kristiansen, Basic Biotechnology, Second Edition, (2001) Cambridge University Press. Bonifacino, Marry Dasso, Joe B.